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Carla Zotti 1 Article
Experimental Study to Develop a Method for Improving Sample Collection to Monitor Laryngoscopes after Reprocessing
Savina Ditommaso, Monica Giacomuzzi, Elisa Ricciardi, Carla Zotti
Clin Endosc 2018;51(5):463-469.   Published online August 21, 2018
AbstractAbstract PDFPubReaderePub
/Aims: The microbiological surveillance of endoscopes and automated flexible endoscope reprocessing have been proven to be two of the most difficult and controversial areas of infection control in endoscopy. The purpose of this study was to standardize a sampling method for assessing the effectiveness of standard reprocessing operating procedures for flexible fiberoptic laryngoscopes (FFLs).
First, the sampling devices were directly inoculated with Bacillus atrophaeus spores; second, tissue non tissue (TNT) wipes were tested on artificially contaminated surfaces and on FFLs.
Comparison of the sponges, cellulose, and TNT wipes indicated that the TNT wipes were more effective in releasing spores (93%) than the sponges (49%) and cellulose wipes (52%). The developed protocol provides a high efficiency for both collection and extraction from the stainless steel surface (87% of the spores were removed and released) and from the FFL (85% of the spores were removed and released), with relatively low standard deviations for recovery efficiency, particularly for the analysis of the FFL.
TNT wipes are more efficient for sampling surface areas, thereby aiding in the accuracy and reproducibility of environmental surveillance.


Citations to this article as recorded by  
  • A cost comparison between reusable flexible and disposable laryngoscopes
    James Ellis, Albert H Park, Aaron Prussin
    American Journal of Otolaryngology.2022; 43(2): 103321.     CrossRef
  • Using Microbiological Sampling to Evaluate the Efficacy of Nasofibroscope Disinfection: The Tristel Trio Wipes System in Ear–Nose–Throat (ENT) Endoscopy
    Savina Ditommaso, Monica Giacomuzzi, Raffaella Cipriani, Teresa Zaccaria, Rossana Cavallo, Valeria Boggio, Roberto Albera, Carla M. Zotti
    International Journal of Environmental Research and Public Health.2019; 16(22): 4583.     CrossRef
  • What is the Best Sampling Method to Monitor the Effect of Endoscopy Reprocessing?
    Kwang Hyun Chung, Byung Ik Jang
    Clinical Endoscopy.2018; 51(5): 397.     CrossRef
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